Figure 1. Effect of IL-13 on ROS Production in MN9D Cells.

(A) MN9D cells were treated with 1, 5 or 10 ng/ml IL-13 for 5 or 60 min or 24 hr. ROS levels were determined using CM-H₂DCFDA as described in Methods. Results are presented as the relative fluorescence compared to untreated cells. All of the experiments were done in sextuplicate and repeated at least three times. (B) MN9D cells were treated with 80 μM H₂O₂ or 2.5 μM tBOOH alone or in the presence of 1, 5 or 10 ng/ml IL-13. ROS levels were determined using CM-H₂DCFDA as described in Methods. Results are presented as the relative fluorescence compared to H₂O₂- or tBOOH-treated cells. All of the experiments were done in sextuplicate and repeated at least three times.