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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Cytokine. 2017 Sep 30;103:114–120. doi: 10.1016/j.cyto.2017.09.018

Figure 2. Effects of IL-13 and Oxidative Stress on Stat6 and Akt Phosphorylation.

Figure 2

MN9D cells were untreated or treated with 10 ng/ml IL-13, 80 μM H2O2 or 2.5 μM tBOOH or a combination of either IL-13 and H2O2 or IL-13 and tBOOH for the indicated times. Cell lysates were prepared and equal amounts of protein were analyzed by SDS-PAGE and immunoblotting with antibodies to phospho and total Stat6 and phospho and total Akt. (A) – (C) Western blots from three independent experiments similar to the ones shown in (D) and (E) were scanned and quantified. (D) and (E) Representative Western blots. *** p < 0.001 relative to control cells. ^^ p < 0.01; ^^^ p < 0.001 relative to IL-13-treated cells