Figure 1.

Overview of the aging reporter. Yeast cells are transformed with the plasmid YCplac111 harbouring the GFP molecule under control of the HO endonuclease promoter (a). Each time the yeast cells divide, GFP molecules are produced at the transition from the G1 to S‐phase. In this way replicative aged yeast cells have a much brighter GFP fluorescence than young cells (b). Using elutriation centrifugation old cells (bright GFP fluorescence) are separated from young cells (low GFP fluorescence). The GFP fluorescence gives a direct feedback to the age of yeast cells and can be used to screen for substances as well as genes that prolong the replicative lifespan (c)