Figure 3.

Sorting of GFP high cells from yeast cells transformed with an aging reporter. Cells were first gated on a FSC/SSC plot to exclude dead cells and debris (a). Subsequently, single cells were gated based on FSC‐H/FSC‐W (b). Yeast cells transformed with the GFP‐reporter plasmid showed 0.05% of GFP‐high cells (c), a 10‐fold higher amount compared with yeast cells transformed with the control plasmid (d) [Colour figure can be viewed at wileyonlinelibrary.com]