. Author manuscript; available in PMC: 2018 Feb 12.

Published in final edited form as: Biometals. 2015 Feb 8;28(3):541–551. doi: 10.1007/s10534-015-9830-3

Table 1.

Diameter of growth inhibition zones (mm) in the agar diffusion antibacterial susceptibility assay.

Compds	Gram-positive bacteria			Gram-negative bacteria
	B. subtilis	S. aureus	M. luteus	P. aeruginosa		E. coli	A. baumannii
	B. subtilis	S. aureus	M. luteus			E. coli	A. baumannii
	ATCC 6633	SG 511	ATCC 10240	K799/wt	K799/61	X580	ATCC 17961^a
Ciprofloxacin	28^c	28^b	12P^b	21^c	24/32p^c	31^d	15p^b
13	34	25	14	27/31P	31/46P	34/38P	NT
24	26	22	0	18P	19/26P	32/39P	NT
23	20/26p	13P	0	19	19/26p	27	20
25	0	0	0	14	0	21	18

p, partially clear inhibition zone/colonies in the inhibition zone; P, unclear inhibition zone/many colonies in the inhibition zone; NT, not tested

Exactly 50 µL of a 0.2 mM solution of each compound dissolved in 1:9 DMSO/MeOH was filled in 9 mm wells in agar media (Mueller Hinton II). Inhibition zones read after incubation at 37 °C for 24 h.

A. baumannii was tested in the presence of 100 µM of 2,2’-bipyridine.

^b,c,d

Ciprofloxacin was tested at (b) 5 µg/mL, (c) 1.66 µg/mL, (d) 0.33 µg/mL in H₂O.