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. Author manuscript; available in PMC: 2018 Feb 12.
Published in final edited form as: Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2015 Apr 23;7(6):759–778. doi: 10.1002/wnan.1346

FIGURE 7.

FIGURE 7

Mesoporous silica nanoparticles (SNPs) for cell labeling and differentiating induced pluripotent stem cells (iPSCs). (a) Positively charged mesoporous SNPs were functionalized with an HNF3β-plasmid-DNA (pHNF3β) and delivered to iPSCs. The treated iPSCs showed differentiation capacity and differentiated into hepatocyte-like cells with mature functions within 2 weeks. (b) Comparing uptake efficiency of different charged mesoporous SNPs shows that positively charged SNPs have greatest uptake. (c) Immunofluorescence analysis for the expression of hepatic markers HNF3β (green) and HNF4α (red) in iPSCs treated with loaded mesoporous SNPs. 1/16 and 1/128 refer to ratios of delivery (scale bar = 200 μm). (Reprinted with permission from Ref 59. Copyright 2013 American Chemical Society)