Table 4.
Resting values of inflammatory biomarkers and their changes with mental stress according to MSIMI status (MIPS Study).
| MSIMI
|
Unadjusted
|
Adjusted Model 1**
|
Adjusted Model 2†
|
Adjusted Model 3‡
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Negative (n = 508) Median (IQR) |
Positive (n = 99) Median (IQR) |
β (95 % CI) | P value* | β (95 % CI) | P value* | β (95 % CI) | P value* | β (95 % CI) | P value* | |
| A) Resting inflammatory biomarkers | ||||||||||
| IL-6, pg/mL | 1.4 (1.0–2.1) |
1.3 (1.0–2.1) |
0.01 (−0.19, 0.21) |
0.91 | 0.01 (−0.18, 0.20) |
0.93 | 0.03 (−0.14, 0.20) |
0.74 | −0.02 (−0.20, 0.16) |
0.87 |
| MCP-1, pg/mL | 122.9 (101.3–147.1) |
123.5 (107.0–147.9) |
0.05 (−0.04, 0.14) |
0.27 | 0.05 (−0.03, 0.14) |
0.20 | 0.06 (−0.02, 0.13) |
0.16 | 0.05 (−0.03, 0.13) |
0.22 |
| MMP-9, ng/mL | 61.6 (40.6–97.1) |
61.0 (44.9–93.1) |
0.08 (−0.12, 0.28) |
0.42 | 0.09 (−0.11, 0.28) |
0.38 | 0.08 (−0.11, 0.28) |
0.39 | 0.08 (−0.12, 0.29) |
0.43 |
| Hs-CRP, mg/L | 1.6 (0.6–3.7) |
1.4 (0.7–5.1) |
0.19 (−0.22, 0.61) |
0.36 | 0.19 (−0.20, 0.57) |
0.35 | 0.14 (−0.27, 0.56) |
0.50 | 0.10 (−0.38, 0.55) |
0.67 |
| B) Mental stress induced inflammatory responses§ | ||||||||||
| IL-6, pg/mL | 0.3 (0.02–0.7) |
0.2 (−0.04 to 0.8) |
−0.06 (−0.19, 0.07) |
0.37 | −0.05 (−0.17, 0.08) |
0.47 | −0.02 (−0.15, 0.10) |
0.74 | −0.02 (−0.15, 0.10) |
0.75 |
| MCP-1, pg/mL | 4.9 (−4.1 to 15.4) |
7.4 (−2.7 to 16.1) |
0.01 (−0.05, 0.06) |
0.81 | 0.01 (−0.05, 0.06) |
0.84 | 0.01 (−0.04, 0.07) |
0.65 | 0.03 (−0.03, 0.09) |
0.27 |
| MMP-9, ng/mL | 5.0 (−8.3 to 20.7) |
7.3 (−5.4 to 24.7) |
0.02 (−0.09, 0.14) |
0.68 | 0.02 (−0.10, 0.13) |
0.76 | 0.01 (−0.10, 0.13) |
0.81 | 0.002 (−0.12, 0.12) |
0.98 |
| Hs-CRP, mg/L | −0.01 (−0.1 to 0.08) |
−0.01 (−0.2 to 0.04) |
−0.06 (−0.14, 0.02) |
0.13 | −0.07 (−0.15, 0.01) |
0.08 | −0.04 (−0.12, 0.05) |
0.39 | −0.02 (−0.10, 0.07) |
0.70 |
Abbreviations: MSIMI, mental stress-induced myocardial ischemia; IL-6, interleukin-6; MCP-1, monocyte chemoattractant protein-1; MMP-9, matrix metallopeptidase 9; HsCRP, high-sensitivity C reactive protein.
Data was presented as median (25th and 75th percentiles). A log 2 transformation was used for biomarker values.
p values were obtained from mixed linear models.
Adjusted model 1: Adjusted for age, sex, African American race, body mass index, smoking, diabetes, hypertension, beck depression inventory score, and previous myocardial infarction.
Adjusted model 2: Adjusted model 1 + accounting for biomarker plate as a random effect.
Adjusted model 3: Adjusted model 2 + adjusting for sequence of mental/physical stress.
Responses are calculated as the differences between 90-min post mental stress values and resting values. Inflammatory response was modeled as the outcome adjusted for resting levels.