Extended Data Figure 9. Lack of cytotoxic activity by pathogen-specific T-cells against autologous lymphoma cells.

(Left) CD4 T-cells were purified from patient MCL030 after autologous tumor vaccination. T-cells were stimulated with autologous PBMCs loaded with either a pool of pathogenic peptides including antigens derived from CMV, EBV, influenza A, and tetanus. After 30 hours, cells were sorted for CD137 expression. (Right) Sorted pathogen antigen-specific cells were expanded using anti-CD3, anti-CD28, IL2, and allogeneic feeders for 3 weeks. The expanded T-cells were co-cultured with fluorescently-labeled autologous lymphoma cells for 24 hours (Top right). Background cell death of the lymphoma cells is also shown (Bottom right). Cytotoxicity of lymphoma cells was determined by 7-AAD uptake of the labeled population.