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. Author manuscript; available in PMC: 2018 Feb 12.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Sep 3;478(4):1706–1712. doi: 10.1016/j.bbrc.2016.09.008

Fig. 3. POSTN expression in various mesenchymal cells and BM cells.

Fig. 3

(A) Quantitative PCR. Postn mRNA was reduced in Med1−/− MEFs compared with Med1+/+ MEFs, and was not restored in Rev-Med1−/− MEFs (left panel). Postn was variably expressed in BM stromal cells and BM cells (right panel). The values are plotted as the fold increase versus the value in Med1+/+ MEFs (left panel) or MS-5 cells (right panel).

(B) ELISA of culture media. POSTN was secreted by various mesenchymal cells and BM cells.

(C–E) Quantitative PCR. MS-5 cell Postn mRNA was prominently induced when cocultured with BM cells (C). MS-5 cell or OP-9 cell Postn mRNA was induced when cocultured with MB-1 cells for 24 h (D). When cocultured for 24 h in transwell to inhibit direct contact between BM cells and MS-5 cells, Postn mRNA was not induced (E).

(F) ELISA of culture media. mPOSTN secretion was induced by physical interactions of MS-5 or OP-9 cells with BM cells or MB-1 cells.

N = 3 (A–F).