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The selection of the working concentration of W-donor bead and anti-W polyclonal antibody (counts are means of triplicates).
| Conjugated compound W–photosensitive microparticles | Anti-W polyclonal antibody |
|||
|---|---|---|---|---|
| 1:500 | 1:1000 | 1:2000 | 1:10 000 | |
| 1:10 | 168 232 | 99 801 | 59 291 | 44 479 |
| 1:100 | 80 573 | 42 568 | 25 180 | 21 516 |
| NSB | 10 | 9 | 11 | 11 |
Abbreviation: NSB, nonspecific binding.
