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The selection of biotinylated goat anti-rabbit IgG antibody dilution (counts are means of triplicates).
| Biotinylated goat anti-rabbit IgG antibody dilution | 1:100 | 1:200 | 1:300 | 1:400 | 1:500 | 1:600 |
|---|---|---|---|---|---|---|
| Zero concentration net luminescent signal | 36 172 | 30 179 | 26 517 | 10 532 | 5432 | 2371 |
| NSB | 85 | 52 | 21 | 11 | 6 | 5 |
Abbreviations: IgG, immunoglobulin G; NSB, nonspecific binding.
