Table 1. MALDI analysis of PK-resistant fragments in recMoPrPSc-950.
| recMoPrPSc-950 | ||||
|---|---|---|---|---|
| Observed mass (MALDI) (kDa) |
Theoretical mass (kDa) |
Sequence | Corresponding WB bands |
Equivalent PK-resistant fragment(s) in GPI-anchorless PrPSc |
| 13441.1 | 13431.0 | A116-S230 | ~14.6 kDa | A116/G118- S232* |
| 11871,3 | 11867.1 | S134-S230 | ~13 kDa | M133/S134—S232 |
| 11391,9 | 11395.6 | I138-S230 | ~12 kDa | G141—S232 |
| 10997.2 | 11998.1 | G141-S230 | ~12 kDa | G141-S232 |
| 9514.1 | 9513.6 | N152-S230 | ~10.2 kDa | N152—S232 |
| 9399.5♦ | 9399.5 | M153-S230 | ~10.2 kDa | M153-S232 |
| 8187.9 | 8184.1 | Y162-S230 | ~8 kDa | Y162-S232 |
| 6097.7 | 6103.9 | V179-S230 | ~6.7 kDa | V179-S232 |
*(GPI-) PrP mice (tg44-/-) express PrP with a sequence that includes two extra serine residues at its C-terminus ([26]).
♦Exact value obtained from ESI-TOF analysis (see Materials and methods) and used for internal calibration of MALDI-TOF data.