Table 1. Clonal and non-clonal chromosome aberrations and genome variations at different MSC passages.
| Total passages | Total cells | Karyotype | ||
|---|---|---|---|---|
| 1. GTG | Normal cells | Cells with aneuploidy/ polyploidy |
Cells with structural chromosome aberrations | |
| 6th | 20 | 46,XY[18] | 45,X,-Y 45,XY,-8 |
- |
| 2. mFISH | ||||
| 6th | 32 | 46,XY[25] | 45,XY,-11 45,XY,-12 45,XY,-17 45,XY,-22 |
46,XY,t(X;2)(p21;q14) 46,XY,t(2;7)(p23;p21) 46,XY,t(2;7)(q14;p15) |
| 9th | 40 | 46,XY[31] | 45,XY,-2 47,XY,+6 47,XY,+8 45,XY,-17 45,XY,-21/ 92,XXYY |
46,XY,chrb(1)(p35) 46,XY,chrb(5) 45,XY,-10,del(21)(q22) |
| 12th | 86 | 46,XY[73] | 45,XY,-1 45,XY,-8 45,XY-9[3]/ 91,XXYY,-9 |
45,X,chrb(X)(q25)Y,-8 46,XY,t(2;4)(p24;q12),t(6;12)(q24;q12)2 46,XY,t(2;11)(q12;q14) 45,XY,-3,chtb(7)(p13) 46,XY,t(4;9)(p12;q32) 46,XY,t(10;17)(q22;q21) 46,XY,t(10;21)(q26;q22) |
| 16th | 87 | 46,XY[59] |
45,X,-Y[2] 48,XY,+8,+8[7]1 45,XY[–9] 45,XY,-11 45,XY,-14/ 92,XXYY |
45,X,-Y,t(9;12;18)(q13;p13;p11.2) 46,XY,t(1;8)(q24;p23) 46,XY,t(1;20)(p13;q13) 46,XY,t(1;20)(p32;p12) 46,XY,t(2;6)(q37;p13),t(13;22;16)(q13;q12;q24) 46,XY,t(5;9)(p14;q34) 46,XY,t(5;21;8)(p10;q21;p21) 46,XY,chrb(6)(q22) 45,XY,del(7)(q31),-20 48,XY,+8,+8,chrb(22)(?)1 45,XY,-9,chtb(20)(q12) 46,XY,t(9;13)(q13;q12)4 45,XY,-10,chrb(11)(p11) 46,XY,t(13;18)(q12;q12) 46,XY,t(13;22)(p11;q13) |
| 18th | 60 | 46,XY[40] | 45,X,-Y 45,XY,-3 45,XY,-4 48,XY,+8,+8[9]1 45,XY,-12/ 92,XXYY |
46,XY,chtb(2)(q35) 46,XY,t(2;4)(p24;q12),t(6;12)(q24;q12)2 45,XY,-6,chtb(5) 46,XY,t(8;14)(?;q32) 46,XY,t(9;13)(q13;q12)4 46,XY,del(11)(p12;q23) |
| 20th | 61 | 46,XY[41] |
45,X,-Y[5] 45,XY,-7 48,XY,+8,+8[2]1 45,XY,-13 45,XY,-22 |
46,XY,t(1;5)(p36.3;p12) 46,XY,t(1;15)(q21;q22) 46,XY,t(2;4)(p24;q12),t(6;12)(q24;q12),t(6;21)(p24;q22)2 45,XY,t(3;16)(q28;q11),t(9;12)(p13;q13),-12 46,XY,del(6)(q21),t(6;14)(?;q32),del(12)(q13)3 91,XXYY,del(6)(q21),-del(6)(q21),t(6;14)(?;q32)x23* 46,XY,t(6;21)(q11;p11.2) 46,XY,t(6;22)(?;q13) 46,XY,del(7)(q31) 46,XY,del(14)(q24) |
| 22th | 40 | 46,XY[27] | 45,XY,-1 45,XY,-4 45,XY,-10 45,XY,-12 47,XY,+19/ 90,XXYY,-8,-8 92,XXYY[2] |
46,XY,chtb(5)(q14) 46,XY,chrb(5)(?) 46,XY,del(6)(q21),t(6;14)(q?;q32)3 46,XY,t(8;17)(q23;q21),-12 46,XY,t(14;20)(q31;?) |
The number of cells is given in square brackets
1 –clone 1
2 –clone 2
3 –clone 3
4 –clone 4
*—clonal chromosome aberration in the polyploid cells.