Figure 7.

Discovery and biosynthesis of the nemamides. (a,b) Comparison of average peak areas for metabolite features in wild-type worms versus pks-1 mutant worms (a) and in wild-type worms versus nrps-1 mutant worms (b), with nemamide A and B highlighted in red. (c) Chemical structures of nemamide A and B. (d) Proposed biosynthetic assembly line for the nemamides. Domain abbreviations: acyl transferase (AT), acyl carrier protein (ACP), ketosynthase (KS), ketoreductase (KR), dehydratase (DH), methyltransferase (MT), aminotransferase (AMT), adenylation (A), peptidyl carrier protein (PCP), condensation (C), and thioesterase (TE). Domains labeled with an asterisk are predicted to be inactive based on the nemamide structures. Figure is adapted.⁹³