Table 3. Plasmids used in this study.
| Plasmid | Product | System | Ind | Res | Source |
|---|---|---|---|---|---|
| pVS52 | CheZ-5G-YFP | pBAD33 | ara | cam | Sourjik and Berg, 2002a |
| pVS149 | CheY-5G-mRFP1 | pTrc99a | IPTG | amp | Sourjik and Berg, 2002a |
| pSJAB12 | CheZ-5G-YFP/CheY-5G-mRFP1 | PTrc99a | IPTG | amp | This work |
| pSJAB106 | CheZ-5G-YFP/CheY-5G-mRFP1¶ | PTrc99a | IPTG | amp | This work |
| pVS91 | CheB† | pTrc99a | ara | cam | Liberman et al., 2004 |
| pVS97 | CheB-D56E‡ | pBAD33 | ara | cam | Clausznitzer et al., 2010 |
| pVS112 | CheBc§ | pBAD33 | ara | cam | V. Sourjik |
| pSJAB 122 | CheBc-GS4G-mVenus | pBAD33 | ara | cam | This work |
| pSJAB 123 | CheB(D56E)-GS4G-mVenus | pBAD33 | ara | cam | This work |
| pSJAB 124 | CheB-GS4G-mVenus | pBAD33 | ara | cam | This work |
| pZR 1 | FliC* | pKG116 | NaSal | cam | This work |
| pPA114 Tsr | Tsr | pPA114 | NaSal | cam | Ames et al., 2002 |
¶Contains a A206K mutation to enforce monomerity..
†expresses WT CheB.
‡carries a point mutation D56E in CheB.
§expresses only residues 147–349 of CheB, preceded by a start codon (Met).
*expresses sticky FliC filament (Scharf et al., 1998).