Figure 1.

Phylogenetic tree of AtVIP1, its orthologues from representative plant species and Arabidopsis homologues from the same subgroup. AtVIP1, its six closest Arabidopsis thaliana homologues and the Nicotiana tabacum orthologue are highlighted by shaded boxes. The evolutionary history was inferred using the neighbour‐joining method (Saitou and Nei, 1987). The optimal tree with the sum of branch lengths = 3.60596289 is shown. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches (Felsenstein, 1985). The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method (Zuckerkandl and Pauling, 1965) and are in the units of the number of amino acid substitutions per site. The analysis involved 38 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 179 positions in the final dataset. Evolutionary analyses were conducted using the Molecular Evolutionary Genetics Analysis tool (MEGA, version 6.0.5 for Mac OS) (http://www.megasoftware.net) (Tamura et al., 2013), which also generated this description of the analysis. Scale bar, 0.05 amino acid substitutions per site.