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. 2018 Feb 12;8:2814. doi: 10.1038/s41598-018-21098-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expanded TM cells are multipotent progenitors. Using P3 cells cultured on 2D Matrigel as a control, P3 cells reseeded in 3D Matrigel were subjected to a number of differentiation assays. TM cells cultured on 2D Matrigel and in 3D Matrigel could be induced into HCEC-like cells (A) and adipose cells (B). However, TM cells cultured in 3D Matrigel could but those on 2D Matrigel could not be induced to chondrocytes (B). Both cells could not be induced to keratocytes (C). Nuclear counterstaining by Hoechst 33342 (A,C). Scale bars: 50 µm (B) and 20 µm (A,C).