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. 2018 Feb 12;8:2785. doi: 10.1038/s41598-018-20973-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CD44 depletion influences intracellular levels of amino acids in Min6 cells. Min6 cells transfected with control or CD44 (#2) siRNAs and incubated in the presence of 5 or 25 mM glucose for 1 h were subjected to metabolome analysis by CE-MS. The amounts of amino acids (nmol/mg protein) were determined. Amino acids are shown classified according to their acidic (yellow frame), neutral (green frame), or basic (blue frame) nature. The abbreviations for amino acids transported into cells by LAT1 are shown in red. ND, not detected. Data are means ± s.d. for three independent experiments. *P < 0.05, **P < 0.01 (Tukey’s HSD and F test).