Figure 2.

AKL1 overexpression suppresses the rate of hyphal elongation. (A) Wild-type cells containing a URA3 vector plasmid (KC840) or a URA3 MAL2-AKL1 plasmid (KC841) were grown overnight in YEP + 2% raffinose, then diluted in YEP + 2% maltose at 37°C, with or without 10% serum, as indicated. Samples were retrieved and fixed at the indicated times and the proportion of germ tubes was determined by microscopic observation. Six hundred cells were counted in each culture. (B) Length of germ tubes was measured in the same cultures as for (A). Hundred cells were measured for each condition. The differences between each vector and MAL2-AKL1 pair were all highly significant by Student's t-test (p < 10⁻⁴).