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. 2018 Feb 8;8:17. doi: 10.3389/fcimb.2018.00017

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Copyright © 2018 Bar-Yosef, Gildor, Ramírez-Zavala, Schmauch, Weissman, Pinsky, Naddaf, Morschhäuser, Arkowitz and Kornitzer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CaAkl1 is a repressor of fluid-phase endocytosis. (A) Homology tree of the Polo and Ark/Prk kinases of S. cerevisiae and C. albicans aligned using T-COFFEE (Di Tommaso et al., 2011). (B) LY uptake in wild-type cells overexpressing AKL1 under the MAL2 promoter (KC 817) vs. vector plasmid (KC816). (C) LY uptake in wild-type (KC274) cells, the AKL1^+/− heterozygote (KC553), the akl1^−/− deletion homozygote (KC554), akl1^−/− with vector plasmid (KC824) or with AKL1 plasmid (KC825). Overnight cultures were shifted from YEP + 2% raffinose to YEP + 2% maltose (B) or diluted in YPD (C) and incubated at 37° for 2.5 h before carrying out the assays. The error bars indicate the standard deviation between three independent cultures.