Figure 8.

Effect of Akl1 and Pan1 on HSG expression. The strains used were the akl1^−/− deletion together with a vector plasmid (KC824), or wild-type with a vector plasmid (KC868), with PAN1 under the MAL2 promoter (KC869), with AKL1 under the MAL2 promoter (KC817), or with both AKL1 and PAN1 under the MAL2 promoter (KC863). Overnight cultures were diluted in YEP-2% maltose and grown for 5 h at 37°C. Two independent cultures were assayed for each strain. The bar graph depicts the average of the two cultures. The Northern blots are shown in Supplementary Figure 8.