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. 2018 Feb 12;8:2867. doi: 10.1038/s41598-018-21072-y

Figure 4.

Figure 4

Gal-3 activated the JNK and NF-κB pathways and played an important role in the inflammatory activity of P.g.-LPS. (A) Trophoblasts (HTR-8 cells) were stimulated with rhGal-3 (0.5 μg/ml), and cell lysates were examined by immunoblot analysis. Phosphorylation of JNK, c-Jun, ERK and p65 was evident. (B) HTR-8 cells were stimulated by different concentrations of rhGal-3 (0, 0.5, 2.5 μg/ml) for 48 h. The mRNA-expression levels of COX-2, Gal-3, IL-8, and TNF-α were analysed by PCR and real time PCR. The expression of cytokines were markedly upregulated. (C) TNF-α production from rhGal-3 (1 μg/ml)-stimulated HTR-8 with or without CAPE (1 μg/ml) for 4 h, pre-exposed to dicumarol/U0126 (10 μM) for 30 min were measured by ELISA after 2 days. Data are presented as the mean ± SD. *P < 0.05; **P < 0.01; 1-way ANOVA. The experiments were performed at least 3 times with similar results.