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. 2018 Feb 12;8:2798. doi: 10.1038/s41598-018-21105-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of PANDAR-protein complexes. (a) eGFP fused protein candidates were analyzed with respect to their PANDAR binding ability by RT-qPCR. Bound PANDAR RNA was determined as fold-enrichment compared to eGFP negative control (=1). Three positive PANDAR binding proteins candidates (dark red bars) exceed the MAD of the population by a factor of >3 (z-score > 3), median = 177, MAD = 168. (b) Analysis of bound proteins to biotinylated PANDAR RNA. Western blot analysis for each of the binding candidates revealed binding of SAF-A, PTBP1 and U2AF65, but not for RBMS2 (z-score = 1) as internal negative control. In: input, FT: flowthrough, B: bound fractions.