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. 2018 Feb 12;8:2808. doi: 10.1038/s41598-018-20724-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nemopilema nomurai jellyfish venom (NnV) inhibits the migration and invasion of HepG2 cells. (A) Cells were plated in a 24-well cell culture plate and treated with various concentrations of NnV. Cell viability was measured by MTT assays. (B) Confluent monolayers of HepG2 cells were scratched with a pipette tip, treated with NnV, and photographed under a light microscope. (C) Migration activity was quantified with ImageJ software. (D) Invasion of HepG2 cells was measured using Boyden’s Transwell assays. Data are presented as the mean ± standard deviation (SD) from three observed fields. *p < 0.01 compared to the control.