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. 2018 Feb 12;8:2808. doi: 10.1038/s41598-018-20724-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nemopilema nomurai jellyfish venom (NnV) suppresses the expression of epithelial–mesenchymal transition (EMT)-related markers and transcription factors. Cells were treated with NnV at different doses (A) for different periods (B). (C–H) Expression levels of EMT markers and regulators were quantified using ImageJ. (I) Cells were treated with NnV, followed by immunostaining for E-cadherin (green) and vimentin (red), and counterstaining with DAPI (blue). Data are presented as the mean ± SD from three fields. *p < 0.01 compared to the control.