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. 2018 Feb 8;9:95. doi: 10.3389/fphar.2018.00095

FIGURE 2.

FIGURE 2

miR-30a prevents upregulation of Beclin-1 in ox-LDL-stimulated VECs. (A) Dual-luciferase gene reports experiments used to verify Beclin-1 was target gene of miR-30a in VECs. The data were expressed as mean ± SEM, n = 3. ANOVA testing was performed; #P < 0.05 vs. miR-30a mimic group. ΔΔP < 0.01 vs. miR-30a mimic and 3′ UTR wild type (WT) group. (B) The levels of miR-30a were detected by SYBR Green PCR. VECs were transfected with miR-30a mimic and inhibitor, after ox-LDL-stimulated VECs for 24 h, then expression of miR-30a was measured by SYBR Green PCR. (C) VECs viability was detected by MTT. (D–F) SYBR Green PCR showing the expression of Beclin-1 mRNA in VECs and western blotting showing the expression of Beclin-1, LC3 protein in VECs. MiR-30a mimic or inhibitor was transfected into VECs, after ox-LDL-stimulated VECs for 24 h. Data were expressed as mean ± SEM, n = 3. ANOVA testing was performed; ##P < 0.01, #P < 0.05; vs. control group. ΔΔP < 0.01 vs. ox-LDL group.