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. 2018 Feb 8;9:95. doi: 10.3389/fphar.2018.00095

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Copyright © 2018 Li, Yang, Wu and Dai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Paeonol increases miR-30a levels in ox-LDL-stimulated VECs. (A) The levels of miR-30a in VECs ox-LDL were detected by SYBR Green PCR. VECs pretreated with different concentrations of paeonol (30, 60, 120 μM) for 24 h, and then the VECs during their treatment with liquid medium contain ox-LDL (100 mg/L) for another 24 h (B,C). Western blotting showing the expression of Beclin-1 protein and SYBR Green PCR showing the expression of Beclin-1 mRNA in VECs. Values are expressed as mean ± SEM, n = 3. ANOVA testing was performed; ^#P < 0.05, ^##P < 0.01 vs. control. ^ΔP < 0.05; ^ΔΔP < 0.01 vs. ox-LDL group.