Figure 2.

Direct detection of m6A and m5C methylations on genomic DNA of S. acidocaldarius using specific antibodies. The presence of m6A (A) and m5C (B) methylations were investigated by dot blot experiments. Genomic DNA extracted from S. acidocaldarius (gDNA) was added together with a negative control, C⁻ (WGA), and a positive control, C⁺ (WGA methylated in vitro by Dam methyltransferase), in (A) and genomic DNA extracted from H1299 cells in (B). A quantification of the ECL signal corresponding to the detection of m6A, including error bars indicating the standard deviation, is presented in association with representative dot blot results of three independent experiments below (A). The dot blot shown in (B) is representative of results obtained for two independent experiments. Methylene blue staining of DNA was considered as a loading control.