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. 2018 Feb 8;9:137. doi: 10.3389/fmicb.2018.00137

Table 1.

Motifs and associated methylations present along the genomic DNA of S. acidocaldarius.

Motif Nber of motif in the genome Nber of recognition sequences in the genome Type of methylation % of methylated motif
5′-GGCC-3′ 1,140 570
Inline graphic
m4C 97
5′-AGATCC-3′ 1,022 1,022
graphic file with name fmicb-09-00137-i0002.jpg 1,022 graphic file with name fmicb-09-00137-i0003.jpg m6A 24
578 289
Inline graphic
m6A 60.9
5′-GATC-3′ 2,622 1,311
Inline graphic
m6A 32.1

The SMRT sequencing revealed the presence of three motifs: 5′-GGCC-3′, 5′-AGATCC-3,′ and 5′-GGATCY-3′ with “Y” being a pyrimidine (“T” or “C”). The two latter motifs contain 5′-GATC-3′ as core motif (underlined in both) which can be studied as such. Modified nucleobases, for each motif, are written in bold. The number of motifs in the whole genome of S. acidocaldarius, the number of corresponding recognition sequences formed in the genome, the type of methylation and the percentage of methylated motifs are given in this table.