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. 2018 Feb 12;8:2836. doi: 10.1038/s41598-018-20929-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of the primary GBM cell cultures propagated in serum-free and serum containing media. (A) Cells growing in serum-free medium as gliomaspheres, (B) cells growing in serum-free medium on laminin, (C) adherent cell growing in serum containing medium, (D) detection of CD133 in two independent serum-free medium cultures, (E) differentiation of serum-free medium cultured cells induced by 10% fetal calf serum, (F) western blot analysis of Sox-2 and α-tubulin and (G) qRT-PCR of Sox-2 expression in GBM cells propagated in serum-free (DMEM/F12) and serum containing (DMEM + FBS) media. The P value signifies the statistical significance of the difference between the paired primary cell lines as assessed by the Wilcoxon paired test.