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. 2018 Feb 12;8:2836. doi: 10.1038/s41598-018-20929-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hierarchical clustergram discriminating paired primary GSC (yellow) and non-stem glioblastoma cell cultures (blue) propagated in serum-free and serum containing medium, respectively. (A) Based on 51 differentially (P < 0.001) expressed miRNAs, (B)Based on 9 miRNAs differentially (P < 0.0001) expressed in GSC and non-stem cell cultures contained in subclusters IA and II, respectively. A gradient of green and red colors is used in the heatmap (green color indicates lower expression whereas red color indicates higher expression of individual miRNAs in analyzed samples).