Table 1.

Random 6 amino acid peptide library.

Sample	Total reads	Passed filter (%)	Unique peptides	Mean count	Median count	Min-Max
Unselected	12,327,473	89.8	5,536,697	2.00	1	1–144
	13,296,736	89.7	5,791,291	2.06	1	1–170
Thrombin	12,334,072	89.7	5,324,398	2.08	1	1–254
	12,240,464	90.0	5,316,032	2.07	1	1–225
ADAMTS13	11,166,160	89.1	5,211,441	1.91	1	1–74
	12,076,701	89.9	5,379,005	2.02	1	1–71

The results of the high throughput sequencing data analysis pipeline are shown for two samples of the unselected random 6 amino acid peptide library, and following two selections of this library by thrombin and ADAMTS13. The table shows the total number of sequencing reads per sample (total reads), the percentage of reads that passed the quality filters (passed filter) and the total number of unique peptides that were ultimately identified (unique peptides). Also shown is the average number of sequencing counts for each unique peptide (mean count), the median number of counts for each peptide (median count), and the range of counts for each unique peptide (min-max).