Figure 6.

Accumulation of CD8α⁺ dendritic cells (DCs) in the liver in response to challenge with infectious or radiation-attenuated sporozoites (RAS) (10K each) after priming. (A) Immunization strategy. Mice were primed with 50K RAS, on day 15 one group of mice were challenged with 10K infectious sporozoites (Inf. Spz) and another group with 10K RAS. Mice were sacrificed 72 h post-challenge, (B) Accumulation of CD8α⁺ DCs in the liver of mice post-challenge. (C) Panel showing accumulation and activation status of CD8α⁺ DCs with the expression of various costimulatory molecules (CD80, CD86, and CD40), (i) CD80, (ii) CD86, and (iii) CD40. Each bar shown in graph is mean of four individual mice per group. Percent cell shown is the percent out of total mononuclear cells or lymphocytes. N = 4 mice per group. Data are the mean ± SEM. The data were analyzed with non-parametric Mann–Whitney U-test. p < 0.05 is considered as a significant. Data shown represent one of two independent experiments.