Figure 7.

Accumulation of activated CD4⁺ T cells and CD8⁺ T cells in the liver on challenging the immunized mice, (A) immunization strategy and cell markers used to characterize CD4 and CD8 T cells. Mice were primed with 50K radiation-attenuated sporozoites (RAS), on day 15 one group of mice were challenged with 10K Inf. Spz and another group with 10K RAS. Mice were sacrificed 72 h post-challenge, (B) accumulation of activated CD4⁺ T cells in liver, (i) % IFN-γ-producing CD4⁺ T_E/EM cells, (ii) % CD40L expressed CD4⁺ T_E/EM cells. (C) Accumulation of activated CD8⁺ T cells, (i) schematics of IFN-γ⁺ and CD107a⁺ CD8⁺ T_E/EM cells, (ii) % IFN-γ-producing CD8⁺ T_E/EM cells, (iii) % CD107a expressed CD8⁺ T_E/EM cells, (iv) % CD40L expressed CD8⁺ T_E/EM, (v) % CD40L expressed CD8⁺ T_CM. Each bar shown in graph is mean of four individual mice per group. Percent cell shown is the percent out of total mononuclear cells or lymphocytes. N = 4 mice per group. Data are the mean ± SEM. The data were analyzed with non-parametric Mann–Whitney U-test. p < 0.05 is considered as a significant. Data shown represent one of two independent experiments.