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. 2018 Feb 12;8:2786. doi: 10.1038/s41598-018-21134-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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C. crescentus titration. 10,000 TZM-bl cells were incubated for 48 hours with 200TCID₅₀ HIV-1_89.6 and differing amounts of recombinant C. crescentus (Cc-Control, Cc-Elafin, Cc-BmKn2, Cc-A1AT or Cc-Indo). HIV-1 infection was measured using a β-galactosidase assay. To minimize assay-to-assay variability in OD₄₁₅ values, the control wells containing HIV-1 + TZM-bl cells with the background of TZM-bl cells subtracted out were set as 100% infection. To obtain the percent infection values for the experimental wells (HIV-1 + TZM-bl cells + Cc-Control or HIV-1 + TZM-bl cells + recombinant C. crescentus) the OD₄₁₅ values were normalized to the 100% infection (HIV-1 + TZM-bl cells) value. Each experiment was performed in quadruplicate and repeated at least three times.