Fig. 1.
Mesenchymal p55TNFR signals are necessary for the development of TNF-mediated arthritis. a Representative histological images of haematoxylin & eosin (H&E)-stained ankle joint sections of hTNFtg p55TNFRf/f (n = 5, age: week 5; n = 8, age: week 10) and hTNFtg p55TNFRMs-KO mice (n = 8, age: weeks 5 and 10). b Representative histological images of H&E-stained ankle joint sections of p55TNFRf/f and p55TNFRMs-KO mice subjected to CAIA at the peak of disease (Day 8). c Representative scoring of clinical manifestations of p55TNFRf/f (n = 7) and p55TNFRMs-KO mice (n = 8) subjected to CAIA (total 3 experiments, n = 20 mice per genotype). Arthritis was induced in male mice by i.v. injection of 4 mg collagen II antibody cocktail (Ab) on Day 0 followed by 75 μg LPS i.p. 3 days later. Scale bar: 1 mm. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001 by two-tailed Student’s t-test