Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 12;8:2821. doi: 10.1038/s41598-018-21121-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Induction of differentiation of IHCC organoids to functional hepatocytes. (a) Schedule for hepatocyte differentiation using IHCC organoids. IHCC organoids were cultured for 7–10 days in EM additionally supplemented with BMP7. Then, the culture medium was changed to DM. DM was changed every 2–3 days for a period of 12 days. GSEA was performed using a ‘hepatocyte differentiation’ gene set. IHCC organoids cultured in DM have a strongly enriched hepatocyte differentiation signature (p < 0.01, FDR < 0.01). (b) Relative expression of Albumin (ALB), CYP3A4 and HNF4A in IHCC organoids cultured in EM or DM. (c) Albumin secretion, CYP3A4 activity and bile acid production in IHCC organoids cultured in EM or DM. (d) Relative expression of Albumin (ALB), CYP3A4 and HNF4A in IHCC organoids cultured in EM, or in EM without R-spondin 1 (Rspo).