Figure 1.

Leucine-rich repeat kinase 2 (LRRK2) is expressed in dendritic cells and is downregulated by Aspergillus fumigatus. (A) LRRK2 mRNA in D1 cells. Data is displayed as the mean gene expression ± SD of two biological replicates and normalized to the housekeeping protein, GAPDH. Differences found to be statistically significant by one-way ANOVA with Bonferonni’s Multiple Comparison post-test are indicated (NS, non-significant; ****, p < 0.0001). (B) Protein expression level of LRRK2 in whole cell lysates of D1 cells stimulated with A. fumigatus condia and swollen conidia. For densitometry analysis, band densities for LRRK2 were normalized to the band density of the housekeeping protein, GAPDH, after which the density of the LRRK2 band of the treated sample was compared with that of the untreated sample. Data is representative of three independent experiments. Abbreviations used: Untreated (Unt); A. fumigatus conidia (A-con); A. fumigatus swollen conidia (A-sw).