Table 1.

Impact of osmotic stress and rewatering on a set of physiological parameters

Parameters	Unit	Treatment
		Osmotic stress phase		Rewatering
		Control (Ctrl)	Stressed (St)	Control (Ctrl)	Stressed (St)
Projected leaf area	cm2	46.3 (11.6)	26.8 (7.8)	81.1 (20.1)	44.4 (17.1)
Leaf expansion rate	cm2 days−1	3.1	1.4	5	2.5
Rosette dry weight	mg	132 (32)	106 (34)	302 (88)	175 (52)
Rosette growth rate	mg days−1	10.3	7.9	24.4	9.9
Root dry weight	mg	25.6 (1.6)	23.1 (7.0)	53.8 (8.9)	33.2 (14.0)
Root growth rate	mg days−1	2	1.7	4	1.5
Shoot–root ratio DW	mg shoot mg−1 root	5.1	4.6	5.6	5.3
Water content	g water g−1 fresh weight	0.92 (0.01)	0.89 (0.02)	0.92 (0.01)	0.91 (0.01)
Leaf osmotic potential	Mpa	− 0.88 (0.06)	− 1.33 (0.48)	− 0.9 (0.11)	− 0.92 (0.12)

The measurements (except growth rates) are performed 11 days after the onset of osmotic stress (D + 35) and 7 days after the beginning of rewatering (D + 42). Growth rate, leaf growth rate, and root growth rate are calculated during the osmotic stress phase (D + 24 to D + 35) and the rewatering phase (D + 35 to D + 42). DW is obtained from 31 (day 24 after sowing) and 26 plants (day 35 and 42 after sowing) per condition [control (Ctrl) and stressed (St)] from three independent experiments. Root dry weight is obtained from 11 (day 24 after sowing), 16 (day 35 after sowing) and 20 plants (day 42 after sowing) per condition (Ctrl and St) from three independent experiments. The projected leaf area is determined using picture of plants from three experiments, with the threshold color plugin of ImageJ software. Around 120 plants per condition [control (Ctrl) and stressed (St)] are used for day 24 and day 35 after sowing, and 70 for day 42 after sowing. Water content is determined from 15 whole rosettes per condition from three independent experiments. Osmotic potential is measured on three-to-five excised leaves per plant on 15 plants from three independent experiments. Three measurements are performed for each sample