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. 2018 Feb 12;8:2823. doi: 10.1038/s41598-018-20421-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nanosight analysis of mRPC released extracellular vesicle diameters and concentrations. (A–C) Nanometer diameter ranges and concentrations of mRPC released EVs were analyzed from conditioned media at 12, 24 and 48 h, respectively. Mean diameter for three samples at each time point analyzed were: 12 h = 133+/−3.2 nm, 24 h = 130+/−0.6 nm and 48 h = 132+/−1.8 nm. (D) Sample 3D plot showing EV size/relative intensity at 24 h. (E) Arrows showed light-scattering of individual EVs from a single frame of Nanosight tracking analysis at 24 h. (F) The average concentration of EVs/ml recorded at 0 h = 0.13+/−0.07 E8, 12 h = 3.41+/−0.34 E8, 24 h = 4.91+/−0.35 E8 and 48 h = 5.93+/−0.22 E8.