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. 2018 Feb 12;8:2823. doi: 10.1038/s41598-018-20421-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SEM and TEM characterization of mRPC surface and released extracellular vesicle ultrastructure. (A) SEM analysis of mRPC ultrastructure with extracellular vesicle structures apparent on the soma and left extended process, scale: 2 μm, (B) higher magnification of (A), scale: 1 μm. (C) released EVs isolated from conditioned media using ultracentrifugation appear as clusters under SEM analysis, scale: 300 nm., (D) higher magnification of (C) allows for measurement of two vesicles at 57.15 nm, scale: 100 nm. (E) Immunogold TEM of isolated EV populations reveal the presence of the EV marker CD63, scale: 500 nm, (F) higher magnification of (E), scale: 100 nm.