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. 2018 Feb 12;8:2823. doi: 10.1038/s41598-018-20421-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sucrose-gradient and immunohistochemical characterization of extracellular vesicles. (A) CD63 positive bands were detected in fractions at gradient-densities of 1.12–1.17 g/cm³. (B–F) Immunohistochemical analysis EVs: (B) GFP+ mRPCs (green), (C) anti-CD63 labeling in cytoplasmic and nuclear regions (red), (D) DAPI (nuclei, blue) and (E) overlay. Scale: 10 µm. (F) 3D confocal reconstruction of labeled mRPCs revealed the presence of CD63 positive EVs in the cytoplasm, emerging from lipid bilayer regions of cell soma and on proximal and distal processes.