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. 2018 Feb 12;8:2864. doi: 10.1038/s41598-018-21142-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chemical shift perturbation analysis of Telo24 with RGG3. (A) The imino proton regions of 1D ¹H-NMR spectra of Telo24 either alone (top) or with RGG3 in the ration of 1:1 (bottom). Imino proton resonances were assigned according to the previous report¹⁹, and are indicated by residue numbers. (B) Chemical shift perturbation of each imino proton resonance of Telo24 upon addition of an equivalent amount of RGG3. (C) Schematics illustration of the Telo24 G-quadruplex¹⁹. The guanine residues that exhibited large chemical shift perturbation are highlighted in black.