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Miles assay. Oral (40 mg/mL in drinking water) N(G)-nitro-l-arginine methyl ester (L-NAME), begun 2 days earlier, strikingly reduces extravasation of Evans blue dye (EBD)-albumin complex in nude mouse flank skin after intradermal injection of 100 ng of vascular endothelial growth factor (VEGF), as observed macroscopically or by EBD quantification. ∗∗P < 0.01 versus control (U-test). HBSS, Hanks' balanced salt solution.
