Figure 3.

Effects of combretastatin CA4 on adenovirus expressing vascular endothelial growth factor (VEGF)-A (Ad-VEGF-A¹⁶⁴)–induced mother vessels (MV). Five days after injection of 10⁷ plaque-forming units of Ad-VEGF-A¹⁶⁴ nude mice were treated with combretastatin CA4. A and B: MV exhibit endothelial cell (EC) necrosis (asterisks) and red blood cell extravasation (red arrows) 2 hours after i.p. injection of 6.25 mg of combretastatin CA4. Pink shading demarcates vascular lumens. C: At 6 hours, MV are obliterated and replaced by focal hemorrhage, fibrin, and inflammatory cells. Scale bars: 10 μm (A–C).