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. 2017 Nov;187(11):2590–2601. doi: 10.1016/j.ajpath.2017.07.021

Figure 3.

Figure 3

Effect of a high-fat diet (HFD) and IL-12 deficiency on cytokine expression in the ischemic hind limb. A and B: p40 and p35 relative mRNA levels, as assessed by real-time quantitative PCR in ischemic hind-limb muscle. C: Interferon (IFN)-γ relative mRNA level in wild-type (WT), p40−/−, and p35−/− mice fed a control diet or an HFD. D: Tumor necrosis factor (TNF)-α relative mRNA level in WT, p40−/−, and p35−/− mice fed a chow diet or an HFD. E–H: Relative mRNA levels for markers for M2 macrophages in WT, p40−/−, and p35−/− mice fed a chow diet or an HFD. Arginase-1 (Arg1; E), IL-10 (F), C-type lectin domain containing 10A (Clec10a; G), and Mannose receptor C-type 1 (MRC1; H) mRNA levels. All cytokine gene expression levels were normalized to β-actin. Data are expressed as means ± SEM. n = 5 to 6 (A–H). ∗P < 0.05 versus WT; †P < 0.05 versus WT HFD or WT; ‡P < 0.05 versus WT HFD.