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. 2017 Nov;187(11):2590–2601. doi: 10.1016/j.ajpath.2017.07.021

Figure 7.

Figure 7

Effect of p40 and p35 deficiency on IL-12 signaling, survival pathway, and proangiogenic factors in ischemic muscle limb. Representative immunoblots and densitometric analysis of the expression of phosphorylated STAT4 (P-STAT4; PY693; A and B), total STAT4 (T-STAT4; A and C), phosphorylated Akt (P-Akt; serine 473; D and E), total Akt (T-Act; D and F), phosphorylated endothelial nitric oxide synthase (eNOS) (P-eNOS; serine 1177; G and H), total e-NOS (T-eNOS; G and H), and phosphorylated vascular endothelial growth factor receptor 2 (VEGFR2; tyrosine 1175; I and J). β-Actin was used as loading control. Densitometric measurements were performed to evaluate the fold increase in protein expression or activity. Data are expressed as means ± SEM. n = 6 (A–J). ∗P < 0.05 versus all groups. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.