Table 2.
Antibodies Used in This Report
| Antibody | Host species | Vendor | Catalog no. | Application | Dilution |
|---|---|---|---|---|---|
| GGNBP2 | Rabbit | Pacific Immunology Corp. (Ramona, CA) | PAC-139 | IHC | 1:1500 |
| Cldauin-11 | Mouse | Santa Cruz Biotechnology (Dallas, TX) | SC-9412 | IF | 1:400 |
| SYCP3 | Rabbit | Sigma-Aldrich (St. Louis, MO) | L9393 | IF | 1:200 |
| SALL4 | Mouse | Sigma-Aldrich | B8434 | IF | 1:400 |
| GATA-4 | Goat | Santa Cruz Biotechnology | sc-69269 | IF | 1:400 |
| CYP17 | Mouse | Cell Signaling Technology (Danvers, MA) | 2107 | IHC | 1:1000 |
| α-Tubulin | Mouse | Cell Signaling Technology | 2102 | IF | 1:5000 |
| α-E-catenin | Rabbit | Cell Signaling Technology | 2123 | WB | 1:1000 |
| β-Catenin | Rabbit | Cell Signaling Technology | 9145 | WB | 1:1000 |
| E-cadherin | Rabbit | Cell Signaling Technology | 9134 | WB | 1:1000 |
| N-cadherin | Rabbit | Santa Cruz Biotechnology | sc-7179 | WB | 1:1000 |
| Afadin | Rabbit | Cell Signaling Technology | 9171 | WB | 1:800 |
| Vinculin | Rabbit | Cell Signaling Technology | 4441 | WB | 1:1000 |
| Nectin-2 | Rabbit | Cell Signaling Technology | 9359 | WB | 1:1000 |
| Nectin-3 | Rabbit | Cell Signaling Technology | 9361 | WB | 1:1000 |
| FAK | Rabbit | Cell Signaling Technology | 3285 | WB | 1:1000 |
| Integrin-β1 | Rabbit | Sigma-Aldrich | SAB4501583 | WB | 1:800 |
| GAPDH | Rabbit | Sigma-Aldrich | 3331 | WB | 1:1000 |
CYP17, 17α-hydroxylase; FAK, focal adhesion kinase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GATA, GATA motif binding factor; GGNBP, gametogenetin binding protein; IF, immunofluorescence; IHC, immunohistochemistry; SALL, spalt like transcription factor; SYCP, syanptonemal complex protein; WB, Western blotting.