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. 2018 Feb 12;8:2797. doi: 10.1038/s41598-018-21143-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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recMgIFN-γ purified by immobilised metal affinity chromatograph is mostly in a dimeric form. (a) SDS-PAGE followed by Coomassie staining for total protein detection (left) and detection of 6His-tagged proteins by Western Blot with an anti-6His monoclonal antibody (right). Lane 1: molecular weight marker (with sizes given to the left - sizes greater than 100 kDa were unreliable when constructing a standard curve); lane 2: flow-through (unbound proteins); lane 3: purified protein(s). (b) Size determination of purified recMgIFN-γ by analytical gel filtration analysis on a pre-calibrated Superdex 75 column. Protein peaks were detected at 230 nm (red) and 260 nm (blue), respectively.