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. 2018 Feb 12;8:2797. doi: 10.1038/s41598-018-21143-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dose-dependent induction of the expression of the target gene Irgb2-b1 by recMgIFN-γ in BVK168 and FMN-R cells. Cells were treated for 24 h with either the negative control protein LEA (200 ng/ml), mouse IFN-γ (200 ng/ml) or recMgIFN-γ (10-fold dilution from 200 to 0.2 ng/ml). The reported mRNA fold induction is relative to untreated cells (set as 1; not shown) and normalised to Ywhaz for reference. Mean ± SEM (n = 3 experiments, each with 2 replicates) are shown. Statistical analysis: One-way Anova followed by Dunnett’s multiple comparisons test (all against the LEA control protein). BVK168 (F(5,12) = 3.75, p = 0.0282) *p = 0.0322, FMN-R (F(5,12) = 21.34, p < 0.0001) ****p = 0.0001, ***p = 0.0004. Note that only statistically significant (p ≤ 0.05) differences were depicted in the figure.